Protein A Resin FF Prepacked Column,1ml x 2

GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column. Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus , binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.   Characteristics Ligand Recombinant Streptococcal protein A expressed in E. coli Number of IgG binding sites per ligand 5 M.W. of ligand Approximately 34 kDa PI of ligand 5.17 Degree of substitution Approximately 5 mg protein A / ml settled resin Static binding capacity > 40 mg human IgG/ ml settled resin Matrix component Agarose, 4% highly cross-linked Average particle size 90 μm (45-165 μm) Storage solution 20% ethanol Storage conditions 2-8 °C Shelf life 18 months when stored unopened Column compatibility The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).