General description
Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.
Application
Protein A-Sepharose was used:
- for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
- in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
- as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
- in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.
Protein A-Sepharose™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.
Quantity
Swelling: 1 g swells to approx. 4 ml.
Physical form
Supplied as lyophilized powder stabilized with lactose and dextran.
Legal Information
Sepharose is a trademark of Cytiva
- UPC:
- 51204100
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- P3391-5G
