Protein G Agarose/Salmon Sperm DNA, 2.5 mL

General description

Recombinant Protein G covalently bound to agarose by cyanogen bromide linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein G agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIP assays).

Recombinant protein G has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein G on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.

Packaging

2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.

Quality

Routinely evaluated in Chromatin Immunoprecipitation for use to pre-clear the supernatant from sodium butyrate treated HeLa cells and subsequent immunoprecipitation of DNA cross-linked to acetylated histones

Physical form

2.5 mL packed beads containing 1 mg sonicated salmon sperm DNA, 2.5 mg BSA and approximately 5 mg recombinant Protein G. Provided as a 50% gel slurry for a final volume of 5 mL per vial. Suspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, containing 0.05% sodium azide. Liquid suspension.

Storage and Stability

1 year at 4°C from date of shipment.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.