Protein G-Coated Microplates, 5 Plate(s)

Protein A coated microplates provide alternative methods for immobilizing antibodies for ELISA and other plate-based assay techniques. These plates are uniformly and stably coated with Protein A. They enable antibodies or pre-estabilished antibody-antigen complexes to be captured to wells of the polystyrene plate for subsequent detection in plate readers. The strategy is useful for a variety of assay situations, but it is generally not suitable for sandwich-type ELISA.> Retain antibody activity, which can be lost when antibodies are immobilized by passive adsorption Orient antibodies for maximum antigen-binding capacity Immobilize antibodies without prior purification Ensure minimal variation (<5% well-to-well) from consistent coating Reduce nonspecific binding because plates are pre-blocked     Binds up to 2.5µg antibody/well (100µL coating volume)