Protein G Resin FF,5ml

GenScript Protein G Resin FF is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin FF is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein G Resin FF. Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA. Storage Store in 20% ethanol at 2°C to 8°C. Do not freeze.   Characteristics Ligand Recombinant Streptococcal protein G lacking the albumin-binding sites expressed in E. coli Number of IgG binding sites per ligand 3 M.W. of ligand Approximately 22 kDa PI of ligand 4.69 Degree of substitution Approximately 2 mg protein G/ml settled resin Static binding capacity ≥ 20 mg human IgG/ml settled resin Matrix spherical 4% highly cross-linked agarose Average particle size 90 μm (45-165 μm) Recommended flow rate 50-300cm/h Storage solution 20% ethanol Storage and Stability 18 months when stored unopened at 2-8 °C