General description
Recombinant λ PPase expressed in E. coli. A 221-amino acid, Mn2+-dependent, protein phosphatase. Hydrolyzes phosphate groups on serine, threonine, tyrosine, or histidine residues. Inhibited by orthovanadate. Note: 1KU = 1,000 units.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per min at 30°C, pH 7.8. 20-200 Units will typically remove >95% of phosphates from serine/threonine residues in 0.1 nmol of protein in 30 min. 100-1000 Units will typically remove >95% of phosphates from tyrosine in 0.1 nmol of protein in 30 min.
Physical form
In 100 mM NaCl, 50 mM HEPES, 2 mM DTT, 0.1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
Preparation Note
Further dilute in 50 mM Tris-HCl, pH 7.8, 5 mM DTT, 2 mM MnCl₂, and 100 µg/ml BSA.
Reconstitution
Following initial thaw, aliquot and freeze (-70°C).
Other Notes
Zhuo, S., et al. 1993. J. Biol. Chem.268, 17754.
Gordon, J.A. 1991. Methods Enzymol.201, 477.
Cohen, P.T.W. and Cohen, P. 1989. Biochem. J.260, 931.
Legal Information
Brij is a registered trademark of Croda International PLC
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
recombinant: expressed in E. coli. Quality Level: 100. Assay: ≥. 95% (SDS-PAGE). form: liquid. specific activity: ≥. 400,000 . units/mL. manufacturer/tradename: Calbiochem®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. foreign activity: Protease, none detected. shipped in: wet ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 539514-20KU