Proteinase K: Activity: >30 units/mg protein (hemoglobin, pH7.5, 37oC) Unit definition: It is the amount of enzyme which releases at 37 oC in 1 min as many folinpositive amino acid and peptides from hemoglobin as 1umol of tyrosine. Features: Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF. Usage: In presence of 0.5-1.0% SDS Proteinase K inactivates DNases and RNases in eukaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids. Preparation Instructions: This product is soluble in double distilled water (1 mg/ml), yielding a clear colorless solution. Quality: Purified by chromatography and lyophilized RNases: Not detectable DNases: Not detectable Exonucleases: Not detectable
- UPC:
- 12350000
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- PB0451.SIZE.250mg
