Proteinase K from Tritirachium album limber (C09-1003-300)

Proteinase K (PROK) is a serine protease with broad specificity towards aliphatic, aromatic and other hydrophobic amino acids. PROK has a molecular weight of 27,000 daltons and is Ca2+dependent. It is not inactivated by metal ion chelating agents such as EDTA, sulfhydryl reagents, PCMB, TLCK, or TPCK. It also retains activity in 0.5% SDS. It can be inhibited by PMSF or DFP.Useful for the proteolytic inactivation of nucleases during the isolation of DNA and RNA. Removal endotoxins bound to cationic proteins such as lysozyme and ribonuclease A. Reported useful for the isolation of hepatic, yeast, and mung bean mitochondria Determination of enzyme localization on membranes Treatment of paraffin embedded tissue sections to expose antigen binding sites for antibody labeling. Digestion of proteins from brain tissue samples for prions in Transmissible Spongiform Encephalopathies (TSE) research. Specification: ≥20 units/mg dry weight MDL Number: MFCD00132129 Molecular Formula: C29H27N2O12P Molecular Weight: 626.5 EC Number: 254-457-8 PubChem CID: 118855431 Isomeric SMILES: C[C@H]([C@@H]1C(=O)N2[C@]1([C@](C(=C2C(=O)OCC3=CC=C(C=C3)[N+](=O)[O-])OC4=CC=CC=C4)(C)OC5=CC=CC=C5)OP(=O)(O)O)O Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/P128666-SCI_f4a8657fb369a6730021bf67eedf1be0.pdf