Proteins

The reagent kit adopts a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). Add the sample, standard substance, and HRP labeled detection antibody to the pre coated micropores of adenosine diphosphate (ADP) antibody, then

Human IgG Antibody: Isotype Control Antibody.

Human IgG Antibody: Isotype Control Antibody.

DescriptionAladdin provides purified Human Tyrosyl DNA Phosphodiesterase (Tdp1) purified to homogeneity to a single 68 kDa band on SDS-PAGE.  Tdp1 is catalytically active and will remove a terminal 3’ phosphotyrosyl group from a specifically

DescriptionAladdin provides purified Human Tyrosyl DNA Phosphodiesterase (Tdp1) purified to homogeneity to a single 68 kDa band on SDS-PAGE.  Tdp1 is catalytically active and will remove a terminal 3’ phosphotyrosyl group from a specifically

DescriptionTyrosyl-DNA phosphodiesterase-2  (TDP2) functions to remove trapped topoisomerase II/DNA complexes by processing 5′- blocking protein (topo II) at the site of the DNA break (Fig 1). In terms of physiological relevance, there appear to be

DescriptionTyrosyl-DNA phosphodiesterase-2  (TDP2) functions to remove trapped topoisomerase II/DNA complexes by processing 5′- blocking protein (topo II) at the site of the DNA break (Fig 1). In terms of physiological relevance, there appear to be

Product InfoIncluded Materials:10X assay buffer and dilution buffer is included.  DescriptionHigh purity, catalytically active Top1 Enzyme overexpressed in the baculovirus system and purified to single band homogeneity.  These preparations are

Product InfoQuality Control:The presence of the 170 band is confirmed by Western blotting: less than 5-10% degradation of the 170 band is detected.There is no 180 kDa topo II in this preparation.Loading 10 ul of extract is sufficient for use as a

Product InfoTopoisomerase II Assay ConditionsDecatenation assays are carried out using kinetoplast DNA substrate in a final volume of 20-30 µl in topo II reaction buffer (50 mM Tris-Cl, pH 8.0, 120 mM KCl, 10 mM MgCl2. 0.5 mM ATP, 0.5 mM

Product InfoTopoisomerase II Assay ConditionsDecatenation assays are carried out using kinetoplast DNA substrate in a final volume of 20-30 µl in topo II reaction buffer (50 mM Tris-Cl, pH 8.0, 120 mM KCl, 10 mM MgCl2. 0.5 mM ATP, 0.5 mM

Product InfoTopoisomerase II Assay ConditionsDecatenation assays are carried out using kinetoplast DNA substrate in a final volume of 20-30 µl in topo II reaction buffer (50 mM Tris-Cl, pH 8.0, 120 mM KCl, 10 mM MgCl2. 0.5 mM ATP, 0.5 mM