Pseudomonas Agar Base, 2.5 kg

Pseudomononas Agar Base is a modification of Kings A medium (1) which contains magnesium chloride and potassium sulphate to enhance pigment production. Goto and Enomoto (2) formulated CetriNix supplement for the selective isolation of Pseudomonas aeruginosa from clinical specimens. Lowbury and Collins (3) studied cetrimide as a selective agent. CetriNix supplement suppresses Klebsiella , Proteus and Providencia species. C-F-C Supplement was formulated by Mead and Adams (4) making the medium specific for isolation of Pseudomonas from chilled foods and processing plants, environmental samples and water. This medium is recommended for enumeration of Pseudomonas species from meat and meat products. Examine inoculated plates after 24 hours and 48 hours using both white and UV light. The presence of blue-green or brown pigmentation may be considered as presumptive evidence of Pseudomonas aeruginosa . Alteromonas species may form brown or pink colonies on the medium. Storage and Shelf-life: Store below 30°C in tightly closed container and the prepared medium at 2 - 8°C. Use before expiry date on the label. References: 1. King E.O., Ward M.K. and Raney D.E., 1954, J.Lab and Clin. Med., 44:301. 2. Goto S. and Entomoto S., 1970, Jap. J. Microbiol., 14:65. 3. Lowbury E.J. and Collins A.G., 1955, Clin. Path., 8:47. 4. Mead G.C. and Adams B.W., 1977, Br. Poult. Sci., 18:661.