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PT7-FLAG-2

Catalog No.
C005B-440503
Mfr. No.
P1243-10UG
Mfr. Name
Sigma-Aldrich
Qty/UOM
1
UOM
EA
Price: $725.69
List Price: $806.32

General description pT7-FLAG-2 is a 4.8 kb E .

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General description

pT7-FLAG-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a C-terminal FLAG® fusion protein containing the FLAG epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.

The C-terminal FLAG fusion protein may be detected using Monoclonal ANTI-FLAG?M2, Catalog Number
F3165, and purified using the ANTI-FLAG M2 Affinity Gel, Catalog Number A2220.

Vector Maps and Sequences

Components

  • pT7-FLAG-2 Expression Vector, 10 μg (P6867) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • pT7-FLAG-2-BAP Control Vector 1 μg ( P7117) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

Legal Information

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC

Shipping Information:

Dry Ice Surcharge & Ice Pack Shipments: $40

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UPC:
51314349
Condition:
New
Availability:
3-5 Days
Weight:
1.00 Ounces
HazmatClass:
No
MPN:
P1243-10UG
Temperature Control Device:
Yes

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