PureCol(TM) (C15-1304-096)

General description

PureCol^™ collagen is approximately 97% Type I with the remainder being comprised of Type III collagen. It is soluble atelo-collagen. It contains high monomer content as measured by gel permeation chromatography.

It is ideal for coating of surfaces and providing preparations of thin layers for culturing cells. PureCol^™, lyophilized form is not recommended for the formation of a solid gel. PureCol^™ collagen is provided in user-friendly packaging for use and storage. This product is supplied as a sterile lyophilized powder.

Application

PureCol^™ has been used:

• in collagen gel preparation for the 3D sprouting assay with human umbilical vein endothelial cells (HUVEC) spheroids 
• to coat Costar^® Transwell^® Inserts plates before cell migration assays with bladder smooth muscle cells (BMSC)
• to coat culture plates for collagen degradation assay with breast cancer cell lines

Preparation Note

This product is supplied as a lyophilized powder with 15 mg of bovine collagen. When reconstituted with 5 ml of sterile 0.01 N HCl, a concentration of approximately 3 mg/ml is achieved.

Legal Information

Costar is a registered trademark of Corning, Inc.

PureCol is a trademark of Advanced BioMatrix, Inc.

Transwell is a registered trademark of Corning, Inc.

biological source: protein from bovine hide. Quality Level: 100. sterility: sterile-filtered. Assay: 99.9%. form: lyophilized. packaging: pkg of 15 . mg. technique(s): cell culture | mammalian: suitable. shipped in: wet ice. storage temp.: −. 20°C. Storage Class Code: 11 - Combustible Solids. WGK: WGK 1. Flash Point(F): Not applicable. Flash Point(C): Not applicable.