PureEpi Chromatin Preparation and Optimization Kit

General description

The PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results.

Features & Benefits

• Complete set of materials for up to 12 chromatin preparations
• Optimized streamlined protocol with only a single buffer for sonication, IP, or wash
• Protocols for using cells or tissues
• Compatible with Magna ChIP HiSens and EZ-Magna ChIP HiSens chromatin immunoprecipitation kits.
• Compatible with ChIPAb+ validated antibody and primer sets

Application

Research Category
Epigenetics & Nuclear Function

The PureEpi Chromatin Preparation & Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues.

Packaging

Kit capacity: 12 chromatin preparations

Components

10X Glycine

Nuclei Isolation Buffer

SCW Buffer (Sonication/ChIP/Wash)

Crosslink Reversal Buffer

Protease Inhibitor Cocktail II

Physical form

Two boxes containing all necessary reagents to perform 12 chromatin preparations using 15 cm plates of cultured cells or tissue samples.

Storage and Stability

Upon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.