Pearl Monoclonal IgG Purification Kit, 1L Serum/ 0.2L Ascites

The Pearl™ Monoclonal IgG Purification kit allows for the rapid purification of antibodies from cell culture supernatant and ascites fluid. The Pearl™ IgG Purification Resin binds the high abundant, non-IgG proteins (i.e. albumin) and allows the IgG molecules to pass through in a physiological buffer.The IgG molecules can be stored or used in downstream applications without further clean-up, such as ammonium sulfate precipitation. The Pearl™ Monoclonal IgG Purification kit can be used to purify antibodies direct from cell culture supernatant with less than 10% FBS or can be used with ascites fluid after treatment with the supplied Ascites PreTreat. The Pearl™ Monoclonal IgG Purification kit can purify IgG from ~1L cell culture supernatant or 200ml ascites fluid. Also availlable are complete IgG Purification Kits , which are available in two formats: A spin format that is ideal for the rapid, small scale purification of IgG. The kit is supplied with 3ml Pearl™ IgG Purification Resin, IgG Isolation Buffer and 20 spin columns. Suitable for purifying up to 25mg IgG. A larger format kit is supplied with 25ml Pearl™ IgG Purification Resin and IgG Isolation Buffer and is suitable for the isolation of IgG from ~100ml serum (~200mg 1gG). Features Isolate monoclonal antibodies for ascites fluid or cell culture supernatant Supplied with ascites pretreatment reagent for optimal IgG purification For 1L of cell culture supernatant or 0.2L ascites fluid Applications Monoclonal antibody isolation from ascites fluid or cell culture supernatant