Puromycin dihydrochloride from Streptomyces alboniger (C09-1018-237)

General DescriptionPuromycin is a broad spectrum antibiotic produced by Streptomyces alboniger . It is an analog of the 3′ end of aminoacyl-tRNA. It is an inhibitor of peptidyl transferase and is active in both prokaryotes and eukaryotes. Chemical structure: peptidyl nucleosideApplicationPuromycin dihydrochloride is a part of the amino-nucleoside family of antibiotics and is derived from Streptomyces alboniger . It is a broad spectrum antibiotic with antitumor activity, as an inhibitor of protein synthesis and has been used to study transcription regulatory mechanisms that control the sequential and coordinate expression of genes during cell differentiation. The recommended working concentration in eukaryotic cell culture is 1-10 μg/mL. Puromycin dihydrochloride from Streptomyces alboniger has been used: in the preparation of puromycin stock solution for puromycin sensitivity assay. to select lentivirus-infected cells containing puromycin resistant selection marker. Used as a selective agent for cells that contain the resistance gene puromycin N-acetyl-transferase (PAC). Recommended for use at a range of 1-10 μg/ml. Sterilize stock solution by filtration using 0.22 μm filter then store in aliquots at −20 °C.Other NotesKeep container tightly closed in a dry and well-ventilated place.Keep in a dry place. Specification: powder, BioReagent, suitable for cell culture Molecular Formula: C22H29N7O5·2HCl Molecular Weight: 544.44 EC Number: 200-387-8 PubChem CID: 443311 Isomeric SMILES: CN(C)C1=NC=NC2=C1N=CN2[C@H]3[C@@H]([C@@H]([C@H](O3)CO)NC(=O)[C@H](CC4=CC=C(C=C4)OC)N)O.Cl.Cl Beilstein Registry Number: 3853613 Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/P432999-SCI_c9982d75e19e000dbe1d337880abcba5.pdf