General description
ATP:pyruvate 2-O-phosphotransferase
Pyruvate kinase has a molar mass of 237,000 and exists as a tetramer. Each polypeptide chain of this tetramer has a molar mass of 57,200. The enzyme contains two identical catalytic particles called protomers. Each of these protomers contains two polypeptide chains. Each protomer contains one site each for Mn2+ and phosphoenolpyruvate.
Application
Pyruvate kinase has been used to measure ATPase activity and in the determination of adenylate concentration.
Biochem/physiol Actions
Pyruvate kinase catalyzes the irreversible conversion of P-enolpyruvate and ADP to pyruvate and ATP with the utilization of a proton. The first step is the transfer of phosphate group from P-enolpyruvate to ADP with the formation of bound enolate of pyruvate and ATP. In the second step, a proton is added to enolate to generate the keto form of pyruvate. Apart from this, the enzyme exhibits other activities, such as ATP- and bicarbonate-dependent ATPase, phosphorylation of fluoride and hydroxylamine, ATP-dependent phosphorylation of glycolate, and decarboxylation of oxaloacetate.
Quality
Contaminants: <0.001% GK, <0.002% “NADH oxidaseâ€, and ATPase, each, <0.01% enolase, LDH, and myokinase, each
Physical form
Suspension in 3.2 M ammonium sulfate solution, pH approximately 6
Preparation Note
Activator: PK requires Mg2+ (or Mn2+, Co2+) and K+ (or NH4+, Rb+) for full activity.
Other Notes
For life science research only. Not for use in diagnostic procedures.
- UPC:
- 41142048
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 10128163001