Cell Line Origin
Rat embryonic fibroblasts RSV-33 transformed
Cell Line Description
The R74 line was derived by co-cultivation of 18 day-old Lewis rat embryonic fibroblasts with cells of a chicken positive for Rous sarcoma virus-33 (RSV-33). This cell population is clearly distinguishable from RT1 and R9 as it is characterised by the presence of fibroblastoid cells that tend to round up and form clusters of refractile cells. R74 is also tumourigenic in chickens and rats.
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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biological source: rat embryo. growth mode: Adherent. karyotype: Near diploid. morphology: Fibroblast-like. products: Not specified. receptors: Not specified. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 93120215-1VL
