Immunogen: Purified N-terminal domain of Staphylococcus aureus alpha hemolysin expressed in E.coli (His-tagged). Description: Rabbit polyclonal reactive to S. aureus alpha hemolysin (α-toxin). The antibody detects the antigen produced by S. aureus strains in western blot and ELISA and neutralizes the hemolytic activity of the toxin. Size: 0.5mg Buffer: PBS + 0.01% Sodium Azide Raised in: New Zealand White Rabbits Purification: Protein A Clonality: Polyclonal Relevance: The antibody can be used for detection and neutralization of α-toxin produced by virulent strains of S. aureus. Recommended Dilutions: ELISA: Assay-dependent dilution WB: Assay-dependent dilution; if probing against purified protein, at least 25 ng of pure α-toxin can be detected with 1 ug/ml of the primary antibody combined with a suitable dilution of a secondary anti-rabbit antibody. Toxin neutralization: using a rabbit RBC lysis assay, IC50 of this antibody for neutralization of 1 ug/ml of α-toxin was determined to be 14 ug/ml. IF: Not tested Storage: 2-3 weeks +4°C, -20°C long term Species Reactivity: unknown. Intended for research use only, not for human, therapeutic, or diagnostic applications.
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- UPC:
- 41181868
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 04-0010
- Temperature Control Device:
- Yes
