Cell Line Origin
Rat Bronchial Epithelial Cells (RaBE) Slow Growth
Cell Line Description
Spontaneously immortalised rat bronchial epithelial cell line derived from a 4 month old male Wistar rat. The cell line is basal cell-like expressing cytokeratins 14 and 15. Vimentin negative. This is a slower growing derivative of the cell line RaBE, Sigma Catalogue number. 04100601, adapted to culture medium without growth factors.
Culture Medium
Ham?s F12 + 2mM Glutamine + 5% Foetal Bovine Serum (FBS)
Subculture Routine
Split sub-confluent cultures (70-80%) between 1:100 and 1:800 i.e. seeding at 20,000 cells/25cm2 with 5ml medium or 5x100,000 cells/75cm2 with 20ml, using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Subculture once a week, double volume at medium change
Other Notes
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- UPC:
- 51285806
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 04100602-1VL
- Product Size:
- 1VL
