General description
The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.
Specificity
Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.
Application
The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:
- Cloning of fragments into either plasmid or phage vectors
- Linker ligation
- Recircularization of linear vector DNA
- Generation of libraries
Packaging
1 kit containing 5 components
Unit Definition
Unit Definition: rAPid Alkaline Phosphatase
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.
Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl
Other Notes
For life science research only. Not for use in diagnostic procedures.
usage: sufficient for 160 . reactions (04898125001), sufficient for 40 . reactions (04898117001). Quality Level: 100. manufacturer/tradename: Roche. parameter: 37 °C optimum reaction temp.. storage temp.: −. 20°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 1. Flash Point(F): does not flash. Flash Point(C): does not flash.- UPC:
- 41106300
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 4898117001
