General description

Rappaport Vassiliadis medium is used for the selective enrichment and presumptive detection of Salmonella sp. This medium is a modification of Rappaport-Vassiliadis enrichment broth and is used for motility enrichment in Petri dishes. It is a rapid and sensitive medium for the isolation of Salmonella sp. and can be seeded directly after pre-enrichment.

Application

Rappaport Vassiliadis medium is used for the selective enrichment and presumptive detection of Salmonella sp. in pharmaceutical products.

Analysis Note

Soy Peptone provides nitrogenous, carbonaceous, B complex vitamins and other essential growth nutrients. Phosphate provides buffering capacity to the medium. Malachite green serves as a selective agent. Sodium chloride maintains the osmotic equilibrium of the media. Salmonella generally survives a slightly high osmotic pressure (due to MgCl₂ in the medium), grows at a slightly low pH, and is resistant to malachite green compared to other bacteria, thus making it a selective media for the growth of Salmonella sp.