General description
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RLF from Cell Applications, Inc. have been used to demonstrate that exaggerated (compared to adults) NF-κB activation in neonates in response to hyperoxia is mediated by phosphorylation of IκBα on Tyr42 (Wright, 2009). They were also used to elucidate melatonin effects and signaling pathway (Ben Soussia, 2012) and served as a control in studies investigating epithelial-mesenchimal transition as the source of myofibroblasts responsible for idiopathic pulmonary fibrosis (Willis, 2005; Jain, 2007).
Cell Line Origin
Lungs
Application
Pulmonary function, gene expression, healing & repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development.
Components
Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
Quality
Each lot was tested for proper morphology, Population Doublings, Negative for HIV, Hepatitis B, Hepatitis C, mycoplasma, bacteria, and fungi.
Preparation Note
- 2nd passage, >500,000 cells in Rat Fibroblast Basal Medium that contains 10% FBS and 10% DMSO
- Can be cultured at least 10 population doublings
Subculture Routine
Please refer to the RLF Culture Protocol.
Shipping Information:
Dry Ice Surcharge & Ice Pack Shipments: $40
More Information: https://cenmed.com/shipping-returns
- UPC:
- 51161711
- Condition:
- New
- Availability:
- 3-5 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- MPN:
- R506-05N
- Temperature Control Device:
- Yes
