Cell Line Origin
Rat pituitary adenoma
Cell Line Description
RC-4B/C1 is a clone of the parent RC-4B/C which was established from a 3 year-old CAI/FMaixF344/FMai(F1) male rat pituitary adenoma. Staining studies have shown the cell line contains many bi-hormonal cells with FHSß, LHß and prolactin. Electron microscopy shows the appearance of endogenous C-type rat retroviral particles.
Application
Study of gonadotrophic functions of pituitary gland and regulation of pituitary gonadotrophin gene expression.
Culture Medium
DMEM:EMEM (EBSS) (1:1) + 2mM Glutamine + 0.2mg/ml BSA + 0.5% Non Essential Amino Acids (NEAA) + 5% Nu-Serum (Collab)+ 2ng/ml EGF.
Subculture Routine
Split sub-confluent cultures (70-80%) 1:6 i.e. seeding at 2x10,000 cells/cm2 using 0.25% trypsin/EDTA; 10% CO2; 34°C. Saturation density is reached at 1x 100,000 cells/cm2. Cells may attach lightly and so handling should be kept to a minimum. Care shoul
Other Notes
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biological source: rat brain. growth mode: Adherent. karyotype: Not specified. morphology: Epithelial-like. products: Gonadatrophs. receptors: Gonadotrophin-releasing hormone receptors. technique(s): cell culture | mammalian: suitable. relevant disease(s): cancer. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51421700
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 94020810-1VL
