General description
Ready-To-Go™ T4 DNA Ligase. Catalyzes the formation of a phosphodiester bond between the 5′-phosphoryl group and the 3′-hydroxyl group of two double-stranded DNA fragments. ATP is required for this reaction. Ligation of blunt-ended DNA is greatly stimulated by polyethylene glycol.
Application
Applications include self-circularization of linear DNA prior to transformation, ligation of insert to vector, and ligation of synthetic linkers to blunt-ended DNA. Each predispensed, single-reaction, ambient-temperature-stable tube contains a glassified reaction mix which, when reconstituted to a final reaction volume of 20 μL, yields a solution of reaction buffer, T4 DNA ligase, and ATP.
Features and Benefits
- For covalently-linking DNA fragments in as little as 30 min.
- Single-dose, ambient-temperature-stable reaction mixes.
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Legal Information
Ready-To-Go is a trademark of Cytiva
- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- GE27-0361-01
