Recombinant beta III Tubulin Antibody (C09-0740-257)

Rabbit anti Human Beta III Tubulin Antibody, Recombinant (RR607), could be used for WB, Flow and so on.Application:WB: 1/2000 - 1/5000       Flow: 1/200 - 1/1000Protein FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain. TUBB3 plays a critical role in proper axon guidance and mantainance.Post-translationalSome glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules. Specifications and Purity: Recombinant (RR607); Rabbit anti Human Beta III Tubulin Antibody; WB, Flow; Unconjugated.