General description
Recombinant Enterokinase (rEK) is a highly purified preparation of the catalytic subunit of bovine enterokinase, a serine protease which recognizes the identical cleavage site as the native enzyme (AspAspAspAspLys↓) and has similar enzymatic activity. This enzyme is produced in E. coli and purified to yield the highest activity available, and is qualified for specific cleavage of appropriate fusion proteins. Supplied as a solution in 50% glycerol. EC 3.4.21.9, M.W. 26,300.
Components
Warning
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
Unit Definition
One unit is defined as the amount of enzyme that will cleave 50 µg of fusion protein in 16 hours at 23°C, in a buffer containing 20 mM Tris-HCl pH 7.4, 50 mM NaCl, 2 mM CaCl₂.
Other Notes
Collins-Racie, L.A. et al. 1995. Bio/Technology 13, 982.
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
form: liquid. Quality Level: 200. manufacturer/tradename: Novagen®. . storage condition: OK to freeze, avoid repeated freeze/thaw cycles. storage temp.: −. 20°C. Storage Class Code: 12 - Non Combustible Liquids. WGK: WGK 2. Flash Point(F): Not applicable. Flash Point(C): Not applicable.- UPC:
- 12352204
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 69066-3
