Kex2 is a Ca2+-dependent serine protease and cleaves at C-terminal site of Lys-Arg, Arg-Arg, Pro-Arg in pro-α,factor and killer-toxin precursors maturing, it was discovered in Saccharomyces cerevisiae.But Kex2 can’t recognize and cut a single basic amino acid,such as carboxyl end peptide bond of arginine and lysine. Recombinant Kex2 is a genetically engineered protein expressed in Pichia pastoris and purified by high pressure liquid chromatography. The optimal pH of Kex2 protease is 9.0, and the optimal temperature is 37 ℃. It is stable in buffer (pH 5.0-6.0). The activity of Kex2 is not affected by the conventional serine protease inhibitors such as PMSF, TPCK, TLCK inhibition.Kex2 Protease Recommended Method: Recommended reaction buffer: 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0. If it is not used immediately after dissolution, it is recommended to use 20mM NaAc-HAc, 2mM Ca2+ (pH 5.0~5.5) buffer to dissolve the lyophilized powder. During the reaction, use 5-10 mM Tris, 2mM Ca2+, pH 7.0-9.0 or HEPES, 5mM Ca2+, pH 7.0-9.0 as the reaction diluent.Recommended conditions: Protease: Fusion protein Ratio 1:20~1:1000.
Specifictaions: Carrier Free, GMP, Bioactive, ActiBioPure™, EnzymoPure™, ≥90%(SDS-PAGE), ≥10 units/mg
Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/R141093-SCI_0406d1e31f51f7fb86324940dbec0909.pdf
- UPC:
- 12352200
- Condition:
- New
- Availability:
- 5-10 days
- Weight:
- 1.09 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- R141093-10mg
- CAS:
- 99676-46-7
- Product Size:
- 10mg
