Recombinant LukS-PV (His tagged), 100ug

Description: : Purified, His-tagged Staphylococcus aureus PantonValentine Leukocidin (PVL) S subunit (LukS-PV). The theoretical molecular weight of the protein is 33,713 Daltons including the His-tag. Size: 100 ug of protein is supplied in PBS + 15% Glycerol at a concentration of 1 mg/mL. Protein demonstrates a molecular weight of approximately 30 kDa. Relevance: This protein may be used in functional PVL toxicity assays in combination with LukF-PV, or as a control protein in ELISA assays or Western blotting when detecting LukS in PVL (+) strains of S. aureus. Recommended Dilutions: ELISA: Assay-dependent dilution; internal QC demonstrates detection down to 1 ng/mL of LukS-PV protein when detected with mouse anti-LukS monoclonal antibody (cat # 0221-001) as capture and rabbit anti-LukS polyclonal antibody (cat# 04-0009) as a secondary followed by anti-rabbit horseradish peroxidase conjugate and substrate. WB: Assay-dependent dilution; internal QC demonstrates detection of 1 ng of LukS-PV protein when detected with anti-PVL LukS polyclonal antibody (cat# 04-0009) in Western blotting. PVL Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukF-PV in a concentration range of 1-100 nM. Storage: 2-3 weeks at -20°C, -80°C long term. Intended for research use only, not for human, therapeutic, or diagnostic applications.