Description: : Purified, tag-free Staphylococcus aureus PantonValentine Leukocidin (PVL) S subunit (LukS-PV). The theoretical molecular weight of the protein is 32,465 Daltons Size: 100 ug of protein is supplied in PBS at a concentration of 1.709 mg/mL. Protein demonstrates a molecular weight of approximately 35 kDa Relevance: This protein may be used in functional PVL toxicity assays in combination with LukF-PV, or as a control protein in ELISA assays or Western blotting when detecting LukS in PVL (+) strains of S. aureus Recommended Dilutions: ELISA: Assay-dependent dilution WB: Assay-dependent dilution; internal QC demonstrates detection of 100 ng of LukS-PV protein when detected with antiPVL LukS polyclonal antibody (cat# 04-0009) in Western blotting. PVL Cytotoxicity assay: Cytotoxicity can be detected in human neutrophils when used in combination with LukF-PV in a concentration range of 1-100 nM. Storage: 2-3 weeks at -20°C, -80°C long term. Intended for research use only, not for human, therapeutic, or diagnostic applications.
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- UPC:
- 51473006
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 0540-001
- Temperature Control Device:
- Yes
