Recombinant MCM2 Antibody (C09-0741-847)

Rabbit anti Human MCM2 Antibody, Recombinant (R02-0D3), could be used for WB, IHC, ICC, IF and so on.Application:WB: 1/500-1/1000IHC: 1/50-1/100 ICC/IF: 1/50-1/200 Protein FunctionActs as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.Post-translationalPhosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication. Specification: ExactAb™, Validated, Recombinant, 0.2 mg/mL