Red Blood Cells Polyclonal Antibody, 5mL

Product is the lyophilized powder of rabbit IgG fraction to human red blood cells and buffer salts. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, adjusted to standard titer, filtered through a 0.22 μm filter, vialed and lyophilized. USES: This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers. SPECIFICATIONS: The total protein is measured using the Biuret procedure, with bovine albumin as a standard. Hemagglutination titer is standardized with an in-house control by measurement of agglutination end-point with 10% human red blood cells. Each IgG fraction is tested for purity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected. RECONSTITUTION: Reconstitute product with 20.0 ml of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material.