REDAccuTaq(R) LA DNA Polymerase

General description

REDAccuTaq LA DNA polymerase allows for quick recognition in high throughput applications as well as direct loading of amplification products onto agarose gels for electrophoresis. The inert red dye has no effect on automated sequencing, restriction enzyme digestion, ligation, or other downstream manipulations. However, the PCR product is easily separated from the dye by standard purification methods.

REDAccuTaq^® long and accurate (LA) DNA Polymerase is a blend of Taq DNA polymerase and a small amount of thermostable proofreading polymerase that exhibits 3′→5′ exonuclease and an inert red dye that acts as a tracer and loading buffer. It is suitable for amplifying complex DNA templates like human genomic DNA.

Application

REDAccuTaq^® LA DNA Polymerase produce long DNA amplicons for:

• Mutation analysis
• Cloning genes
• DNA sequencing
• cDNA library generation
• Amplicon sequencing

Features and Benefits

• Increased fidelity, up to 6.5× that of Taq DNA polymerase
• Efficiently and accurately produce amplicons up to 22 kb on genomic templates and up to 40 kb on less complex templates such as lambda or bacterial DNA
• Visual confirmation that enzyme has been added, and proper mixing has occurred
• No additional loading dyes needed. A post-reaction aliquot can be directly used on an agarose gel for electrophoresis

Packaging

The enzyme is provided with an optimized 10× reaction buffer for enhanced amplification of complex templates.

Unit Definition

One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 Â°C.

Other Notes

Store REDAccuTaq LA DNA Polymerase and AccuTaq LA 10x Buffer at –20 °C.

Legal Information

Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,789,224, 5,618,711, 6,127,155 and claims outside the US corresponding to expired US Patent No. 5,079,352. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim, no right to perform any patented method, and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.

REDAccuTaq is a registered trademark of Sigma-Aldrich Co. LLC