General description
REM 134 is a canine mammary carcinoma cell line derived from a spontaneous primary tumour.
Culture Medium
MEM (M2279) + 2mM L-Glutamine (G7513) + 10% FBS (F2442)
Subculture Routine
Split sub-confluent cultures (70-80%) seed new cultures at 2-4 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Citation
If use of this culture results in a scientific publication, it should be cited in the publication as: REM 134 (ECACC 12122002)
- UPC:
- 12352207
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 12122002-1VL