Cell Line Origin
Rat Sprague-Dawley, primary endometrial, SV40 large T antigen transformed
Cell Line Description
Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat. Cells carry vimentin, connexin 43, collagen type III and proenkephalin markers. The transforming agent was SV40 large T antigen.
Culture Medium
DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Doubling time 48 hours.
Other Notes
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biological source: rat endometrium. growth mode: Adherent. karyotype: Not specified. morphology: Spindle shaped. products: Not specified. receptors: Glucocorticoid. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51351603
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 92092512-1VL
