Cell Line Origin
Rat Sprague-Dawley, primary endometrial, SV40 T antigen transformed
Cell Line Description
Derived from the endometrium tissue from the uterus of a 21-28 day-old Sprague-Dawley rat, transformed with a ts mutant of SV40 T antigen (tsA58). Cells express vimentin, CSF-1 (at 39°C), desmin, collagen type III and connexin 43.
Culture Medium
DMEM + 20μg/l Gly-His-Lys (Sigma) + 2mM Glutamine + 80 IU human Insulin/l (Novo) + 10% Foetal Bovine Serum (FBS) (heat-inactivated).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 34°C. Doubling time 48 hours. Cells show continuous growth at 34°C and growth arrest at 39°C.
Other Notes
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biological source: rat endometrium. growth mode: Adherent. karyotype: Not specified. morphology: Cuboidal. products: Not specified. receptors: Glucocorticoid. technique(s): cell culture | mammalian: suitable. shipped in: dry ice. storage temp.: −. 196°C.- UPC:
- 51351705
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 92092516-1VL
