Replacement Wash Buffer, 25ml

Efficient gel extraction and PCR clean-up The Gel/PCR DNA Fragment Extraction Kits are designed to recover or concentrate DNA fragments (50bp-10kb) from an agarose gel, PCR, or any other enzymatic reaction. This method uses a chaotropic salt to dissolve the agarose gel and denature the enzymes. The DNA fragments in the chaotropic salt are bound to the glass-fiber matrix of the spin column. The contaminants are washed with a wash buffer (containing Ethanol) and the purified DNA fragments are eluted by a low salt based elution buffer or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are 80-90% for gel extraction and 90-95% for PCR clean-up. The entire procedure can be completed in 20 minutes, and the eluted DNA is ready to use in restriction digestion, ligation, PCR, and sequencing reactions.