RESIN, G-SEP-50 SUPERFNE, 100G

G-Sep™ is a gel filtration resin comprised of ultrapure cross-linked dextran for desalting and buffer exchange in industrial applications. It exhibits high selectivity, superb resolution, low non-specific adsorption and robust chemical stability. Quickly desalts, removes contaminants and transfers to a new buffer in a single step. Excellent recovery and minimum sample dilution Available in prepacked SpinOUT™ Desalting columns for fast and convenient desalting Molecules purified with G-Sep™ are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Two different G-Sep™ resins are available, G-Sep™ 25 and G-Sep™ 50. They differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. G-Sep™ 25 is better suited for smaller molecules and G-Sep™ 50 is better suited for larger molecules. G-Sep™ 25 and G-Sep™ 50 are both available in 3 different particle sizes (Superfine, Fine, and Medium). The molecular weight cut-off (MWCO) for G-Sep™ 25 is 5 kD for proteins and 10 bases for nucleic acids. The molecular weight cut-off (MWCO) for G-Sep™ 50 is 25 kD for proteins and 20 bases for nucleic acids. Buffer and pH effects on resolution are minimal and purified biomolecules are not significantly diluted (1.5-fold) when processed using G-Sep™. G-Sep™ is autoclavable at 121°C, pH 7 for 30 minutes and is stable in all commonly used buffers, including: 0.2M NaOH; 0.2M HCl; 1M acetic acid; 8M urea; 6M guanidine HCI; 1% SOS, 24% Ethanol; 30% Propanol; and 30% Acetonitrile. Features G-Sep™ 50 Superfine Fine Medium BioProcess resin Yes Yes Yes Matrix Cross-linked dextran Cross-linked dextran Cross-linked dextran Wet bead size 40–100 µm 40-160 µm 100-300 µm Dry bead size 20–50 µm 20–80 µm 50-150 µm Water regain 4.80-5.20 mL/g 4.80-5.20 mL/g 4.80-5.20 mL/g Swelling 9-11 mL/g 9-11 mL/g 9-11 mL/g MWCO (size exclusion) below 25,000 Da below 25,000 Da below 25,000 Da Fractionation range Mr Globular proteins 1,000-30,000 Da 1,000-30,000 Da 1,000-30,000 Da Fractionantion Mp Dextrans 500-10,000 Da 500-10,000 Da 500-10,000 Da pH Stability 2.0-13.0 2.0-13.0 2.0-13.0 Pressure Flow Specification min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d. min 150 cm/h, bed height 10 cm, column 5 cm i.d. min 150 cm/h, bed height 10 cm, column 5 cm i.d. 4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.1 M NaOH Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile Applications Protein purification and contamination removal from samples For the desalting and buffer exchange of protein and nucleic acid solutions Separate proteins from peptides Desalt peptides