RESIN, IMINOBIOTIN, 5ML

Immobilized Iminobiotin Resin consists of iminobiotin, a cyclic guanido analog of biotin, covalently coupled to 6% crosslinked agarose. The resin allows for the purification of avidin, streptavidin and Neutravidin and their subsequent gentle elution using non-denaturing elution buffers. The normal biotin-avidin complex requires strong denaturing buffers, i.e. 8M guanidine•HCl, to denature the avidin and release the biotin, which obviously destroys the native and functional aspects of the avidin. The iminobiotin-avidin complex will form at >pH9.5 and can be dissociated at pH4.0 with gentle elution buffers, including 50mM ammonium acetate, pH4.0 with 0.5M NaCl. Click here to request bulk or custom sizes . Features Biotin Binding Capacity: >2mg avidin/ml resin No requirement for strong, denaturing elution buffers Elutes at pH4.0 Applications Isolation of avidin, streptavidin and Neutravidin complexes