Application
Riboflavin 5′-monophosphate sodium salt hydrate has been used:
- as a component of assay buffer to determine the luminescence of L. lactis cells
- as a component of the reaction mixture in nitric oxide synthase (NOS) enzymatic activity assay
- in the high performance liquid chromatography (HPLC) analysis of Flavin mononucleotide (FMN) cyclase products
- in luciferase assay with firefly luciferase
Biochem/physiol Actions
Riboflavin 5′-monophosphate (FMN), a derivative of riboflavin, is used as a prosthetic group for one and two-electron transfer in oxidation/reduction reactions. It acts as a cofactor for enzymes such as reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase, nitric oxide synthase, and nitrilotriacetate monooxygenase. Riboflavin 5′-monophosphate is used as a substrate to study the specificity and kinetics of FMN phosphohydrolases and to study molecular sensing-based gene regulation by riboswitches.
- UPC:
- 51391611
- Condition:
- New
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- F2253-10MG