Application

Riboflavin 5′-monophosphate sodium salt hydrate has been used:

as a component of assay buffer to determine the luminescence of L. lactis cells
as a component of the reaction mixture in nitric oxide synthase (NOS) enzymatic activity assay
in the high performance liquid chromatography (HPLC) analysis of Flavin mononucleotide (FMN) cyclase products
in luciferase assay with firefly luciferase

Biochem/physiol Actions

Riboflavin 5′-monophosphate (FMN), a derivative of riboflavin, is used as a prosthetic group for one and two-electron transfer in oxidation/reduction reactions. It acts as a cofactor for enzymes such as reduced nicotinamide adenine dinucleotide (NADH) dehydrogenase, nitric oxide synthase, and nitrilotriacetate monooxygenase. Riboflavin 5′-monophosphate is used as a substrate to study the specificity and kinetics of FMN phosphohydrolases and to study molecular sensing-based gene regulation by riboswitches.