RNA Clean Beads (C09-1029-468)

Product DescriptionThe magnetic beads are suitable for rapid sorting and recovery of RNA fragments. Magnetic beads are magnetically separated and washed with ethanol. The high-purity RNA fragments eluted with low-salt elution buffer or nuclease-free water do not contain contaminants such as nucleotides, primers, enzymes, and salts, and can be directly used in downstream applications. For example: sequencing, hybridization, RT-PCR reaction, etc., and can be applied to manual or automatic liquid handling equipment.Features1. The recovery rate can reach more than 80%;2. The operation is fast and simple, and the entire operation process can be completed in 20 minutes without centrifugation.Recommendations1. Inefficient or no RNA recovery?1) RNA is degraded. The operation process should be strictly ensured that there is no RNase contamination.2) The sample quality is low.3) The elution efficiency is low. 80% ethanol needs to be used and prepared immediately. If it is stored for a long time, the concentration may decrease due to the volatilization of ethanol, which will affect the elution effect;4) The incubation time of the elution solution is too short. The magnetic beads should be incubated in the eluent for a specified time, usually 5 min;5) Magnetic beads are over-dried. Do not dry the beads at room temperature for more than 10 minutes, as excessive drying will reduce the elution efficiency.2. Are there magnetic beads remaining in the purified RNA?1) The separation time of the magnetic beads is too short or the magnetic force of the magnetizer is weak. Increase the separation time or choose a magnetizer with strong magnetic force to ensure that the magnetic beads are all adsorbed by the magnetizer;2) During the elution step, the supernatant was aspirated too fast. Aspirate the supernatant slowly, taking care not to aspirate the beads.3. How to check the purity of recovered RNA?It can be assessed by measuring the ratio of A260/A280.Storage ConditionsStore at 4°C.Operating procedures1. Mix RNA and RNase Free Water at a ratio of 1:1.5, mix the diluted RNA sample with 1.8 times the volume of magnetic beads, and let stand at room temperature for 5 minutes;2. Separate by a magnetic device, let stand at room temperature for 5 min, and remove the supernatant by suction;3. Rinse once with 80% ethanol (the sample tube is kept on the magnet during this process);4. Rinse once again with 80% ethanol (the sample tube remains on the magnet during this process);5. Remove the supernatant by suction, let stand for 5 minutes at room temperature, elute with an appropriate amount of RNase Free Water, and let stand for 5 minutes at room temperature;6. Magnetic separation, let stand for 5 min at room temperature, transfer the supernatant to -20 °C for storage. Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/R427280-SCI_fc4efd5a382fdd35320aae3e1f4abee5.pdf