RNA PURIFICATION KIT, 50 PREPS

The GET™ Total RNA kit isolates total RNA from contaminating DNA, proteins, and nucleases using our GET™ RNA Spin Columns. The <60-minute protocol is simple; after homogenization, RNA is bound to the GET™ RNA Spin Columns and washed. The protocol provides an option to remove contaminating DNA with a single DNase treatment. Finally RNA is eluted from the column. The eluted RNA is ready for any procedure including Northern/slot/dot blots, reverse transcription or RNase protective assays. Three step protocol: Arrest™ Extraction Buffer lyses samples instantly destroying RNase activity. GET™ RNA Spin Columns captures RNA. After a brief washing step, pure and protein free RNA is eluted. Features Rapidly inhibit destructive RNases Strong chaotropic buffer for rapid RNA release High affinity RNA binding spin columns Optional DNase treatment Compatible with numerous species and tissues Suitable for animal and plant tissues, cultured cells, blood and bacteria Suitable for 50 x 50mg extractions Applications For the extraction and purification of protein and DNA free RNA.