RNA Sample Loading Buffer (C09-1030-047)

DescriptionRNA loading buffer is used as a tracking dye during RNA electrophoresis. The RNA loading dye has a slight negative charge and will migrate the same direction as RNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:3 to 1:6 with sample, heat to 65C for ten minutes and chill on ice before loading.RNA sample loading buffer is especially formulated for electrophoresis of RNA on formaldehyde-agarose gels with or without ethidium bromide. Ethidium bromide is not recommended for gel staining prior to Northern blot detection because the presence of ethidium bromide in agarose gels or the loading buffer can cause poor transfer efficiency.Suitable for use with formaldehyde-agarose gels used in Northern blotting procedures.RNA Sample Loading Buffer has been used as a sample loading buffer in northern blot. Specification: for NA electrophoresis, with ethidium bromide (50 μg/mL) MDL Number: MFCD00213041 Related Documents: https://ald-pub-files.oss-cn-shanghai.aliyuncs.com/aladdinsci/pdp/sds/1/R485277-SCI_96f6d0ef26a4f9a4de7932e5cc185d4f.pdf