RNA Sample Loading Buffer, with EtBr

Each vial contains 250 μL.|RNA Sample Loading Buffer, with EtBr (ethidium bromide, 50µg/ml) is formulated for use in RNA sample preparation for denaturing formaldehyde-agarose gel electrophoresis. Contains tracking dyes and ethidium bromide, so gel loading buffer and ethidium bromide staining are not required.|Note: This solution contains ethidium bromide which can interfere with transfer of RNA to nylon or nitrocellulose membranes.|Reagent Composition:|62.5 % deionized formamide|1.14 M formaldehyde|1.25X MOPS-EDTA-sodium acetate buffer|200 mg/ml bromophenol blue|200 mg/ml xylene cyanole FF|50 mg/ml ethidium bromideAdd 1 volume sample to 2-5 volumes of sample loading buffer and mix well. The sample should be heated to 65°C for 10 minutes, and then chilled on ice immediately before loading on the gel.RNA Sample Loading Buffer, with EtBris a solution which promotes protein denaturation for use in SDS-PAGE, contains ethidium bromide. Specifications and Purity: Contains ethidium bromide (50 μg/ml).