RNase H is a ribonucleic acid endonuclease that specifically breaks down RNA strands in RNA DNA hybrids, and cannot digest single or double stranded DNA. This product can be used for the detection of DNA-RNA hybrids, removing mRNA during cDNA second strand synthesis, and removing the poly (A) tail of mRNA hybridized to poly (dT).
:Activity definition:
The amount of enzyme required to produce 1 nmol of acid soluble substance within 20 minutes at 37 ° C using poly (rA) · poly (dT) as substrate is defined as 1 active unit (U).
:Usage
:
1．Taking RNA degradation in DNA RNA heterozygotes as an example, configure 20 μ Reaction system.
:
2．React at 2.37 ° C for 30 minutes.Note:This product is a thermally stable RNase H enzyme that still exhibits RNase H activity after incubation at 70 ° C or above for 10 minutes. Specification: 600U
- UPC:
- 12352204
- Condition:
- New
- Availability:
- 8-12 weeks
- Weight:
- 7.05 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- R665576-600U
- Product Size:
- 600U
