RNasin (C09-1564-022)

This product is a recombinant RNase inhibitor expressed and purified in soluble form in Escherichia coli, with a molecular weight of approximately 65 KDa and a broad-spectrum RNase inhibitor. RNasin can specifically bind to RNase in a non covalent bond to form a complex, thereby inactivating RNase, without inhibiting the activity of RNase H, S1 nuclease, SP6, T7 or T3 RNA polymerase, AMV or M-MLV reverse transcriptase, Taq DNA polymerase, RNaseT1 and other enzymes, without affecting subsequent reverse transcription and translation processes. Widely used in RNA research, such as RT-PCR, cDNA synthesis, mRNA protection, in vitro transcription and translation, preparation of RNase Free antibodies, in situ hybridization, and mRNA localization. : : :Component: :R665506 30 μL:R665506 1 ml: : : :RNasin (40 U/μL): :30 μL:1 ml:Storage buffer:HEPES-KOH (pH 7.6) 20 mM, KCl 50 mM, DTT 8mM, Glycerol 50%.Activity definition:One active unit (U) refers to the amount of enzyme used to inhibit the hydrolysis of glycoside 2 ', 3' - cyclic phosphate in 50% 5ng RNase A.Purity:1.300 U of RNasin and 1 μ G's λ The DNA Hind III decomposition product reacted at 37 ℃ for 1 hour without any change in the electrophoresis band of DNA.2.300 U of RNAsin and 1 μ The super helix pBR322 DNA of g reacted at 37 ℃ for 1 hour, and the electrophoresis band of the DNA did not change.3.100 U of RNasin and 1 μ The 16S and 23S rRNAs of g reacted at 37 ℃ for 1 hour, and the electrophoresis bands of the RNA did not change.Main uses:1. cDNA synthesis.2. In vitro translation.3. In vitro transcription.4. RNA amplification.5. RNA purification and storage. :Notes:1. This product should avoid repeated freeze-thaw cycles. Please store it at -70 ℃ for long-term storage.2. It is recommended to use a final concentration of 1 U/ μ L.