ROKbeta/ROCK-I Protein, active, 10 µg (C15-1298-565)

General description

Research area: Apoptosis

N-terminal His6-tagged, recombinant, human ROCK-I amino acids 17-535; autoactivated by incubating with Mg/ATP and redialysed to remove excess ATP

Product Source: expressed by baculovirus in Sf21 insect cells

Application

Research Category
Inflammation & Immunology

Biochem/physiol Actions

G protein, RhoA/Rho kinase 1 (ROCK1) is a serine/threonine kinase. It plays a vital role in the regulation of cell growth, and invasive and metastatic progression of various types of human cancers. ROCK1 is involved in the regulation of apoptosis or programmed cell death in various cell types. Overexpression of ROCK1 has been observed in various tumors, including glioblastoma, melanoma, osteosarcoma, and hepatocellular carcinoma. This protein is known to enhance actin-myosin contractility by phosphorylating regulatory myosin light chains (MLCs). It also plays a role in cell motility through the modulation of integrin β1-activated focal adhesion kinase (FAK) signaling.

Packaging

Also available in 250 µg size --call for pricing and availability and reference catalog number 14-601M when ordering the 250 µg size.

Quality

routinely evaluated by phosphorylation of Long S6 substrate peptide (Catalog #12-420)

Physical form

Ni²⁺/NTA-agarose

Storage and Stability

6 months at -70°C

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.