Rosetta 2(DE3)pLacI Competent Cells - Novagen (C15-1308-191)

General description

Genotype: F^–ompT hsdS_B (r_B^– m_B^–) gal dcm (DE3) pLacIRARE2 (Cam^R)





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Expression host strains for pETBlue and pTriEx vectors are lDE3 lysogens that also carry the compatible pLacI plasmid, encoding the lac repressor. The pLacI plasmid confers chloramphenicol resistance and provides enough lac repressor to inhibit transcription from the T7lac promoter in the absence of inducer. The NovaBlue host strain is recommended for initial cloning with pETBlue and pTriEx vectors due to its superior transformation efficiency and high yields of high-quality plasmid DNA.

Note that these strains are not recommended for use with pET, pACYCDuet, pCDF, pRSF, pCOLADuet or pETcoco vectors.

.Rosetta 2 strains are BL21 derivatives designed to alleviate codon bias when expressing heterologous proteins in E. coli. The cells carry a chloramphenicol-resistant plasmid with the tRNA genes that decode seven rare codons (AGA, AGG, AUA, CUA, GGA, CCC, and CGG) to improve the yield of full-length proteins. The tRNA genes are driven by their native promoters. In Rosetta 2(DE3)pLacI, the rare tRNA genes are present on the same plasmid that carries the lac repressor gene.

DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.

Rosetta strains are BL21 derivatives designed to enhance the expression of eukaryotic proteins that contain codons rarely used in E. coli. Contains the pLacI plasmid producing extra Lac repressor.

Components

0.4 ml1 mlComponent

•2 × 0.2 ml5 × 0.2 mlRosetta 2(DE3)pLacI Competent Cells

•2 × 2 ml4 × 2 mlSOC Medium

•2 ng2 ngTest Plasmid

Warning

Toxicity: Multiple Toxicity Values, refer to MSDS (O)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

biological source: Escherichia coli. Quality Level: 100. manufacturer/tradename: Novagen^®. . storage condition: OK to freeze. growth mode: adherent or suspension. morphology: rod shaped. technique(s): microbiological culture: suitable. cell transformation: transformation efficiency: 2×10⁶ . cfu/μ. g. shipped in: dry ice. storage temp.: −. 70°C. Storage Class Code: 10 - Combustible liquids.