RP-AMIDEC16 5UM 15CMX4.6MM DISCOVERY HPLC COLUMN (C15-1304-279)

General description

Discovery^® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C₁₆-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.

Application

Discovery RP-AmideC16 HPLC Column may be used for the separation of:

• Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).
• Vitamin B₆ compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).
• Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.
• Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.

Discovery^® RP-AmideC16 HPLC column may be used for the separation of:

• Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).
• Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.
• Hydrophilic bases by LC with principal component analysis (PCA).
• 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.

Features and Benefits

• Excellent retention and resolution of polar compounds
• Unique selectivity compared to C18
• Excellent peak shape and efficiency
• Less hydrophobic than C18 columns
• Compatible with 100% aqueous mobile phases

   

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Legal Information

Discovery is a registered trademark of Sigma-Aldrich Co. LLC

Agency: suitable for USP L60. Quality Level: 100. feature: endcapped. manufacturer/tradename: Discovery^®. . extent of labeling: 11% Carbon loading. parameter: ≤. 70 °C temp. range, 400 bar pressure (5801 psi). technique(s): HPLC: suitable. L × I.D.: 15 . cm × 4.6 . mm . surface area: 200 . m²/g . surface coverage: 2.6 . μ. mol/m². impurities: <. 10 . ppm metals. matrix: silica gel, high purity, spherical base material. matrix active group: amide, alkyl phase. particle size: 5 . μ. m. pore size: 180 . Ã…. operating pH range: 2-8. application(s): food and beverages. separation technique: reversed phase. Storage Class Code: 13 - Non Combustible Solids. WGK: WGK 3. Flash Point(F): Not applicable. Flash Point(C): Not applicable.