RPMI 1640 is suitable for a wide range of anchorage independent cells. RPMI 1640 Medium is commonly supplemented with 10% Fetal Bovine Serum (FBS). With 2.0 g/L sodium bicarbonate With HEPES buffer Applications RPMI-1640 medium was developed by Moore et al., at Roswell Park Memorial Institute, hence the acronym RPMI. The formulation is based on the RPMI-1630 series of media utilizing a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. RPMI-1640 medium has been used for the culture of human normal and neoplastic leukocytes. RPMI-1640 when properly supplemented, has demonstrated wide applicability for supporting growth of many types of cell cultures. The original Click formulation was published in 1972 and is a modification of Eagle’s Minimum Essential Medium with Hanks’ salts (HMEM). RPMI 1640: Click;s Medium (1:1) has increased concentrations of many essential amino acids, addition of non-essential amino acids, sodium pyruvate and nucleic acid precursors. Wilson & Horster used the 1:1 mix of RPMI-1640 and Click’s medium to study the differential response of defined distal nephron epithelia in culture. RPMI-1640 Click’s medium is frequently used for the suspension, washing and culture of T-cells.
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- UPC:
- 51441802
- Condition:
- New
- Availability:
- 3 Days
- Weight:
- 1.00 Ounces
- HazmatClass:
- No
- WeightUOM:
- LB
- MPN:
- 112-133-101
- Temperature Control Device:
- Yes
